Culture conditions for mycelial growth of eight Philippine wild strains of tiger sawgill mushroom, Lentinus tigrinus

Authors

  • Sofronio P. Kalaw Center for Tropical Mushroom Research and Development, Central Luzon State University
  • Rich Milton Dulay Center for Tropical Mushroom Research and Development, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Eduardo Damaso Jr. Center for Tropical Mushroom Research and Development, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Joshua Ramos Tuklas Lunas Development Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Mark Adrian Del Rosario Tuklas Lunas Development Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Marcelino Abon Tuklas Lunas Development Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Angeles De Leon Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
  • Renato Reyes Center for Tropical Mushroom Research and Development, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

DOI:

https://doi.org/10.22137/ijst.2023.v7n2.01

Keywords:

Lentinus tigrinus, mycelia, commercial culture media, laboratory made media

Abstract

Lentinus tigrinus is a wild edible and medicinal mushroom with potential culinary and pharmaceutical applications. In the present work, we evaluated the different culture media, pH and physical conditions such as aeration, illumination and temperature for maximum mycelial growth of eight L. tigrinus strains. Mycelial growth of the eight strains respond differently to the nutritional and physical factors. All strains showed maximum mycelial growth on coconut water gulaman (CWG), except TLLt 3, which favored potato dextrose agar (PDA) and malt extract agar (MEA). TLLt 1, 5, 6, 7 and 8 mycelia luxuriantly grew at pH 5.0-8.0 whereas TLLt 2 preferred pH 5.0-7.0, and TLLt 3 and 4 favored pH 5.0-6.5. Sealed condition was found to be more suitable for TLLt 1 mycelia while no significant difference was noted between the two aeration conditions for the mycelial growth of other seven strains. Both TLLt 1 and 2 mycelia favorably grew in lighted condition while the other six favorably grew in both lighted and dark conditions. Room temperature (30⁰C) recorded the fastest mycelial growth fo all strains, but found comparable to air-conditioned temperature (23⁰C) for the growth of TLLt 3, 6 and 8 mycelia. Therefore, mycelial growth of L. tigrinus on the different nutritional and physical parameters may vary depending on the strain type.

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Published

2023-08-31

Issue

Vol. 7 No. 2 (2023): CLSU International Journal of Science and Technology (2023)

Section

Articles