Optimization of Culture Conditions for Mycelial Growth and Basidiocarp Production of Cyclocybe cylindracea (Maire)
DOI:
https://doi.org/10.22137/ijst.2020.v4n1.01Keywords:
Biofuel, Botryococcus spp., Carbohydrate pathwayAbstract
Agrocybe cylindracea is an exotic and unknown species of mushroom among Filipinos. In our intention to develop production technology for this mushroom, the influences of culture media, pH and physical factors such as aeration, illumination and temperature for optimum mycelial growth and fruiting body production were investigated in the present work. Among media evaluated, potato sucrose gulaman, rice bran decoction gulaman and malt extract agar recorded the highest mycelial growth rate (9.00 mm/day), shortest incubation period (10.00 days) with very thick mycelial density. Potato sucrose gulaman (most common culture medium) at pH 6 incubated in either sealed or unsealed, lighted or dark, and in room temperature (28-33°C) or air-conditioned temperature (23-25 ºC) registered the highest mean mycelial growth rate. Corn grit was the best grain spawning material. The substrate formulation of 7:3 rice straw- sawdust ratio recorded the shortest incubation period and primordial initiation, and the highest total yield of 106.94 g. Therefore, A. cylindracea is an addition to the list of exotic edible mushrooms with great potential for cultivation in the Philippines.References
Aminuddin, H., Mohd Khan, A., & Madzlan, K. (2013). Effects of pH on mycelial growth and amino acid composition of Lentinula edodes in submerged cultures. Journal for Tropical Agriculture and Food Science, 41(1), 63–70.
Atlas, R. M. (1997). Handbook of Microbiological Media (2nd ed.). CRC Press.
Barros, L., Baptista, P., Ferreira I. C. F. R. (2006). Influence of the culture medium and pH on the growth of saprobic and ectomycorrhizal mushroom mycelia. Minerva Biotecnologica, 18(4), 165–70.
Bellettini, M. B., Fiorda, F. A., Maives, H. A., Teixeira, G. L., Avila, S., Hornung, P. S., Junior, A. M., & Ribani, R. H. (2019). Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26(4), 633–646.
Borlingame, B., Mouille, B., & Charrondiere, R. (2009). Nutrients, bioactive non nutrients, and anti-nutrients in potatoes. Journal of Food Composition and Analysis, 22, 494–502.
Bulseco, M. G., Abella, E., and Reyes, R. 2005. Morphogenesis of Schizophyllum commune, a wild edible mushroom of Mt. Nagpale, Abucay, Bataan, Philippines. Journal of Nature Studies, 4(1), 20–28.
Bumanlag, C. P. B., Kalaw, S. P., Dulay, R. M. R., and Reyes, R. G. (2018). Optimum conditions for mycelial growth and basidiocarp production of Pleurotus djamor on corn-based media. International Journal of Biology, Pharmacy, and Allied Sciences, 7(4), 558–575.
Bustillos, R. G., Dulay, R. M. R., Kalaw, S. P., & Reyes, R. G. (2014). Optimization of culture conditions for mycelia growth and basidiocarp production of Philippine strains of Panaelus antillarium and Panaelus cyanescens. Mycosphere, 5(3), 398–404.
Carlile, M., & Watkinson, S. (2000). The Fungi (2nd ed.). Academic Press.
Cheung, L. M., Cheung, P. C. K., & Ooi, V. E. C. (2003). Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry, 81, 249–255.
Cuevas, M., Reyes, R., and Kalaw, S. (2009). Biophysiology of Lentinus sajor-caju. Journal of Tropical Biology, 7, 48.
De Leon, A.M., Guinto, L. J. Z. G., De Ramos, P. D. V., & Kalaw, S. P. (2017). Enriched cultivation of Lentinus squarrosulus (Mont.) Singer: A newly domesticated wild edible mushroom in the Philippines. Mycosphere, 8(3), 615–629.
Dulay, R. M. R., Kalaw, S. P., Reyes, R. G., Cabrera, E. C., & Alfonso, N. F. (2012). Optimization of culture conditions for mycelial growth and basidiocarp production of Lentinus tigrinus (Bull.) Fr., A new record of domesticated wild edible mushroom in the Philippines. Philippine Agricultural Scientist, 95(3), 278–285.
Dulay, R. M. R, Arenas, M. C., Kalaw, S. P., Reyes, R. G., & Cabrera, E. C. (2014). Proximate composition and functionality of the culinary-medicinal tiger sawgill mushroom, Lentinus tigrinus (Higher basidiomycetes), from the Philippines. International Journal for Medicinal Mushrooms, 16, 85–94.
Dulay, R. M. R., Cabalar, A. C., De Roxas, M. J. B., Concepcion, J. M. P., Cruz, N. E., Esmeralda, M., Jimenez, N., Aguilar, J. C., De Guzman, E. J., Santiago, J. Q., Samoy, J. R., Bustillos, R. G., Kalaw, S. P., & Reyes, R. G. (2015). Proximate composition and antioxidant activity of Panaeolus antillarium, a wild coprophilous mushroom. Current Research in Environmental and Applied Mycology, 5, 52–59.
Dulay, R. M. R. & Garcia, E. J. B. (2017). Optimization and enriched cultivation of Philippine (CLSU) strain of Lentinus strigosus (BIL1324). Biocatalysis and Agricultural Biotechnology, 12, 323–328.
Dulay, R. M. R. & Rivera, A. G. C. (2017). Mycelial growth and fruiting body production of Philippine (CLSU) strain of Polyporus grammocephalus (BIL7749). Biocatalysis and Agricultural Biotechnology, 11, 161–165.
Dulay, R. M. R., Rivera, A. G. C, & Garcia, E. J. B. (2017). Mycelial growth and basidiocarp production of wild hairy sawgill Lentinus strigosus, a new record of naturally occurring mushroom in the Philippines. Biocatalysis and Agricultural Biotechnology, 10, 242–246.
Eguchi, F., Dulay, R. M. R., Kalaw, S. P., Yoshimoto, H., Miyazawa, N., Seyama, T., & Reyes, R. G. (2014). Antihypertensive activities of Philippine wild edible white rot fungus (Lentinus sajor-caju) in spontaneously hypertensive rats as models. Advances in Environmental Biology, 8, 74–81.
Ferreira, I. C., Vaz, J. A., Vasconcelos, M. H., & Martins, A. (2010). Compounds from wild mushrooms with antitumor potential. Anticancer Agents Medical Chemistry, 10(5), 424–436.
Gibbs, P. A., Seviour, R. J., & Schmid, F. (2000). Growth of filamentous fungi in submerged culture: Problems and possible solutions. Critical Reviews in Biotechnology, 20(1), 17–48.
Girmay Z, Gorems W, Birhanu G, Zewdie S. (2016). Growth and yield performance of Pleurotus ostreatus (Jacq. Fr.) kumm (oyster mushroom) on different substrates. AMB Express, 6(1), 87. https://doi.org/10.1186/s13568-016-0265-1
Oliveira, M. G. D. C., Bassinello P. Z., Lobo, V. L. D. S., & Rinaldi, M. M. (2012). Stability and microbiological quality of rice bran subjected to different heat treatments. Ciência Tecnologia Aliment, 32(4), 725–733. https://doi.org/10.1590/S0101-20612012005000095
Hoa, H. T. & Wand, C. L. (2015). The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(1), 14–23.
Isaac, S. (1995). Mycology answers: moulds, mildews and other fungi are often found in shaded and dark situations- is their development influenced by light?. Mycologist, 9(1), 41–42.
Jacob, J. K. S., Kalaw, S. P., & Reyes, R. G. (2015). Mycelial Growth Performance of Three Species of Pleurotus on Coconut Water Gelatin. Current Research in Environmental and Applied Mycology 5(3), 263–268.
Jasinska, A., Marek, S., & Sobieralski, K. (2012). Mycelium growth and yielding of black poplar
mushroom-Agrocybe aegerita (Brig.) Sing. on different substrates. Journal of Agricultural Science and Technology A, 2, 1040-1047.
Kalaw, S. P., Nabor, R., Dulay, R. M. R., Undan, J. R., Undan, J. Q., De Leon, A. M., &Reyes, R. G. (2017). Mycelia and fruiting body performance of Philippine wild strains of Ganoderma lucidum and Schizophyllum commune on rice bran enriched rice straw-based substrates. International Journal of Biology, Pharmacy, and Allied Sciences 6(6), 1210–1219.
Kalaw, S. P., Alfonso, D. O., Dulay, R. M. R., De Leon, A. M., Undan, J. Q., Undan, J. R., & Reyes, R. G. (2016). Optimization of culture conditions for secondary mycelial growth of wild edible mushrooms from selected areas in Central Luzon, Philippines. Current Research in Environmental & Applied Mycology 6(4), 277–287.
Kalaw, S. P., & Albinto, R. F. (2015). Growth performance and nutritional attributes of Pleurotus species grown on rice straw based formulations. Advances in Environmental Biology, 9(18), 72–81.
Kerekes, J. & Desjardin, D. E. (2009). A monograph of the genera Crinipellis and Moniliophthora from Southeast Asia including a molecular phylogeny of the nr ITS region. Fungal Divers, 37, 101–152.
Klomklung, N., Karunarathna, S. C., Hyde, K. D., & Chukeatirote, E. (2014). Optimal conditions of mycelial growth of three wild edible mushrooms from northern Thailand. Acta Biologica Szegediensis, 58(1), 39– 43.
Kurtzman, R. H., & Martínez-Carrera D. (2013). Review Light, what it is and what it does for mycology. Micologia Aplicada International 25(2), 23–33.
Le, T. H., Nuytinck, J., Verbeken, A., Lumyong, S., & Desjardin, E. D. (2007). Lactarius in northern Thailand: 1. Lactarius subgenus Piperites. Fungal Divers, 24, 173–224.
Leatham, F., & Stahmann, M. A. 1987. Effect of Light and Aeration on fruiting of Lentinula edodes. Transactions of the British Mycological Society, 88(1), 9–20.
Lo, K. M., & Cheung, P. C. K. (2005). Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var. alba. Food Chemistry, 89, 533–539.
Magday, J. C, Bungihan, M. E., & Dulay, R. M. R. (2014). Optimization of mycelial growth and cultivation of fruiting body of Philippine wild strain of Ganoderma lucidum. Current Research in Environmental and Applied Mycology, 4(2), 162–172.
Muruke, M. H. S., Kivaisi, A. K., & Magingo, F. S. S. (2002). Identification mushroom mycelium using DNA technique. Tanzania Journal of Science, 28(1), 115–128.
Muthu, N., & Shanmugasundaram, K. (2017). Effect of five different culture media on mycelial growth of Agrocybe aegerita. International Journal of Pharmaceutical Sciences and Research, 29, 5193–5197.
Neupane, S., Thakur, V., Bhatta, B., Pathak, P., Gautam, B. B., & Aryal, L. (2018). Performance of different substrates on the production of oyster mushroom (Pleurotus florida) at Gokuleshwor, Darchula. International Journal of Scientific and Research Publications, 8(6), 231–240.
Noonsong, V., Puttakun, N., Tinsirisuk, M., & Seephueak, P. (2016). Recycling of spent Pleurotus compost for production of the Agrocybe cylindracea. Mycosphere, 7(1), 36–43.
Nwanze, P. I., Ameh, J. B., & Umoh VJ. 2005. The effect of the interaction of various oil types with different culture media on biomass production of Psathyrellaatroumbonatapegler. African Journal of Biotechnology, 4, 1285-1289.
Obodai, M., Cleland-Okine, J., & Vowotor, K. A. (2003). Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. Journal of Industrial Microbiology and Biotechnology 30, 146–149.
Philippoussis, A., Zervakis, G., & Diamantopoulou, P. (2001). Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World Journal of Microbiology and Biotechnology, 17, 191–200.
Reyes, R. G., Lopez, L. L. M. A., Kumakura, K., Kalaw, S. P., Kikukawa, T., & Eguchi, F., (2009). Coprinus comatus, a newly domesticated wild nutraceutical mushroom in the Philippines. Journal of Agricultural Technology, 5(2), 299–316.
Reyes, R. G., Kalaw, S. P., Dulay, R. M. R., Yoshimoto, H., Miyazawa N, Eguchi F. (2013). Philippine native and exotic species of edible mushrooms grown on rice straw based formulation exhibit nutraceutical properties. Philippine Agricultural Scientist, 96, 198–204.
Sanmee R., Tulloss, R. E., Lumyong, P., Dell, B., & Lumyong S. (2008). Studies on Amanita (Basidiomycetes: Amanitaceae) in Northern Thailand. Fungal Divers, 32, 97–123.
Siddhant S., Yadav, S., & Singh, C. S. (2013). Spawn and spawning strategies for the cultivation of Pleurotus eous (Berkeley). International Journal of Pharmaceutical and Chemical Sciences, 2(3), 326–328.
Sitaula, H. P., Dhakal, R., Geetesh, D. C., & Kakauni, D. (2018). Effect of various substrates on growth and yield performance of oyster mushroom (Pleurotus ostreatus) in Chitwan, Nepal. International Journal of Applied Sciences and Biotechnology, 6(3), 215–219.
Stamets P. (2000). Growing gourmet and medicinal mushrooms (3rd ed.). Ten Speed Press.
Stanley, O. H. & Awi-Waadi, G. (2010). Effect of substrates of spawn production on mycelia of oyster mushroom species. Research Journal of Applied Sciences, 5, 161–164.
Stevens, R. B. (1981). Mycology Guidebook (Vol 27). National Library of Australia.
Tayamen, M.J., Reyes, R., Floresca, E., & Abella, E. (2004). Domestication of wild edible mushrooms as non-timber forest products resources among the Aetas of Mt. Nagpale, Abucay, Bataan: Ganoderma sp. and Auricularia polytricha. Journal of Tropical Biology 3, 49–51.
Tolentino, J. V. (2016). Growth performance and proximate nutrient composition of Pleurotus species on corn based substrate [Unpublished master’s thesis]. Central Luzon State University.
Tsai, S. Y., Tsai, H. L., & Mau, J. L. (2007). Antioxidant properties of Agaricus blazei, Agrocybe aegerita and Boletus edulis. Food Science and Technology, 40, 1392–1402.
Uhart, M., & Albertó, E. (2007). Morphologic characterization of Agrocybe cylindracea (Basidiomycetes, Agaricales) from America, Europe and Asia. Rev. Mexicana Micologia, 24, 9–18.
Utami, C. P. & Susilawati, P. R. (2017). Rice straw addition as sawdust substitution in oyster mushroom (Pleurotus ostreatus) planted media. AIP Conference Proceedings. Vol. 1868.
Wannathes, N., Desjardin, D. E., & Lumyong, S. (2009). Four new species of Marasmius section Globulares from northern Thailand. Fungal Divers, 36, 155–163.
Wannathes, N., Desjardin, D. E., Hyde, K. D., Perry, B. A., & Lumyong S. (2009). A monograph of Marasmius (Basidiomycota) from northern Thailand based on morphological and molecular (ITS sequences). Fungal Divers, 37, 209–306.
Wasser, S. P. & Weis, A. L. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms. International Journal of Medicinal Mushrooms, 1, 31–62.
Watling, R. (1992). Observations on the Bolbitiaceae. Some Brazilian taxa. Boletin Sociedad Argentina de Botanica, 28, 77–103.
Zhao, C., Sun, H., Tong, X., & Qi, Y. (2003). An antitumour lectin from the edible mushroom Agrocybe aegerita. Biochemical Journal, 374, 321–327.
Zhao, R. L., Desjardin, D. E., Soytong, K., Perry, B. A., & Hyde, K. D. (2010). A monograph of Micropsalliota in northern Thailand based on morphological and molecular data. Fungal Divers, 43, 33-–79.
Zhao, R. L., Hyde, K. D., Desjardin, D. E., Raspe, O., Soytong, K., Guinberteau, J., Karunarathna, S. C., & Callac, P. (2011). Agaricus flocculosipes sp. Nov, a new potentially cultivatable species from the palaeotropics. Mycoscience, 53(4), 300–311.
Zharare, G. E., Kabanda, S. M., & Poku, J. Z. (2010). Effects of temperature and hydrogen peroxide on mycelial growth of eight Pleurotus strains. Scientia Horticulturae, 125, 95–102.
Zubano, L. Y., Bellere, A. D., & Savilla, L. C. (2017). Mycelial growth, fruiting body production and proximate composition of Pleurotus djamor on different substrate. The CLSU International Journal of Science and Technology, 2(1), 7–6.